e-learning

Training Custom YOLO Models for Segmentation of Bioimages

Abstract

In bioimages, challenging morphologies are often quite hard to segment using traditional computer vision/image analysis methods. Therefore, using semi-supervised machine learning methods like deep learning for such tasks is getting more popular.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • Why use YOLO for segmentation in bioimage analysis?
  • How can I train and use a custom YOLO model for segmentation tasks using Galaxy?

Learning Objectives

  • Preprocess images (e.g., contrast enhancement, format conversion) to prepare data for annotation and training
  • Perform manual/human-in-the-loop semi-automated object annotation.
  • Convert AnyLabeling annotation files into YOLO compatible format for training.
  • Train a custom YOLO model for segmentation.

Licence: Creative Commons Attribution 4.0 International

Keywords: Imaging

Target audience: Students

Resource type: e-learning

Version: 2

Status: Active

Prerequisites:

  • FAIR Bioimage Metadata
  • Introduction to Galaxy Analyses
  • REMBI - Recommended Metadata for Biological Images – metadata guidelines for bioimaging data

Learning objectives:

  • Preprocess images (e.g., contrast enhancement, format conversion) to prepare data for annotation and training
  • Perform manual/human-in-the-loop semi-automated object annotation.
  • Convert AnyLabeling annotation files into YOLO compatible format for training.
  • Train a custom YOLO model for segmentation.

Date modified: 2025-07-31

Date published: 2025-07-25

Authors: Arif ul Maula Khan, Yi Sun

Contributors: Leonid Kostrykin, Yi Sun

Scientific topics: Imaging


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